Welcome to the Mukhopadhyay group site!
Some news from lab..
March 15th, 2022
New study co-authored by Maren, Megan and Aindrila demonstrating a droplet-based microfluidic system for high throughput strain engineering in collaboration with colleagues from the JBEI’s Technology division is out in Microsystems and Nanoengineering. Congratulations!
Feb 15th, 2022
Undergrad researchers Angela Wei, Kevin Lam and Kevin Chen-Xiao (or Kevin^2 as their mentors call them) from UC, Berkeley joined the group. Welcome and as we get back to normalcy, here’s hoping you have a great time in the lab!
Jan 21st, 2022
Space traveling Cyanobacteria
Aindrila: Hey, What are you up to these days?
Random researcher in the group (RRIG) : Hi Aindrila! I am sending my samples for sequencing today.
Anne Kakouridis: I am sending my samples to SPACE.
RRIG : Wait, what?
Yup, you heard that right!
Anne and Aindrila, in collaboration with Rhodium Scientific and NASA are figuring out ways to transport DNA to space using Cyanobacteria as the model host. Cyanobacteria are fascinating organisms with valuable natural functions such as photosynthesis and viability under extreme conditions. Anne and Aindrila are using these features of cyanobacteria already present in nature to figure out ways to transport DNA to space more easily and reliably.
“In the first part of the project, we transformed simple plasmids into Nostoc punctiforme, desiccated the cultures, and transported them to the International Space Station (ISS) and back. In the second part of the project, we will attempt to revive the cyanobacteria and retrieve the plasmids we placed into them. We will then evaluate if there are any mutations or defects in the DNA to determine if conditions such as desiccation, microgravity, and radiation affected the DNA in situ. Our hope is that the DNA was preserved intact during desiccation and space travel. If this works – these cyanobacteria may become the carrier for DNA for space travel!”, says Anne.
Read the highlights of this work in the biosciences area news and on the ISS national lab website.
Jan 5th, 2022
A new article with our collaborators Douglas Clark, Jay Keasling and John Hartwig exploring the assembly and evolution of artificial metalloenzymes within E. coli cells for enantioselective and site-selective functionalization of C─H and C═C bonds is out in JACS. Congrats to Jing!
Jan 3rd, 2022
A happy new year indeed with new members in the group! We warmly welcome Shawn Kulakowski and Silvia Rossbach to the group.
Shawn recently graduated with a B.A in Biochemistry and Molecular Biology, B.S in Chemical Biology from UC Berkeley and will work on developing syn bio tools for nitrogen-fixing bacteria to create sustainable alternatives to chemical fertilizers.
Silvia is a visiting scientist from Western Michigan University where she works on microbial ecology, understanding the influence of heavy metals on soil bacteria and how plant-bacteria interactions promote plant growth and bioremediation.
Dec 14th, 2021
Thomas Eng is now Deputy Director of Host Engineering. Congrats, Thomas! Don’t forget to check out this cool video where he talks about his exciting research at the lab.
Dec 3rd, 2021
We are thrilled to announce that Aindrila will assume the role of the BSE Deputy for Science. In this role, she will be involved with directing the S&T programs in the BSE division and be an Ambassador for Science internally and externally to the Lab. Congratulations, Aindrila and we look forward to working under your leadership!
Nov 18th, 2021
Our study on genomics characterization of an engineered Corynebacterium glutamicum grown in bioreactor under ionic liquid stress is out in Frontiers in Bioengineering and Biotechnology. Congratulations to all the authors!
Oct 14th, 2021
Of synthetic chemists, biologists and unnatural biosynthesis..
Check out this supercool study published in Nature Chemistry with our collaborators – Douglas Clark, Jay Keasling and John Hartwig. We demonstrate that marrying synthetic biology with synthetic chemistry can produce molecules that were previously inaccessible to nature, in engineered E. coli cells. Congrats to Jing who led this study! This work was also featured in Chemical and Engineering news and the Berkeley News.
Sep 26th, 2021
Hearty Congratulations, Dr. Megan Garber! That was an awesome finishing talk with some supercool analogies. Good luck with your postdoctoral fellowship at UCSF!
June 29th, 2021
Ankita’s cool study on the discovery of Viral Genomes from the circular plasmid sequence data is out in mSystems!
June 25th, 2021
A new review in Nature Microbiology Reviews with Jay Keasling and our close collaborators at JBEI and ABPDU.
May 6th, 2021
Deepanwita and Thomas lead another nice study where we show that a deletion in a response regulator GacA in P. putida results in greater fitness during bioreactor growth. This mutation also improved the production of Indigoidine from the non-sugar carbon source para-coumarate. Read our OA paper here!
March 11th, 2021
We had our 2021 annual m-group offsite virtually this year. It isn’t the same as spending a day together in person but it’s the next best thing! We had some really great activities! Hearty laughing at impromptu presentations, a great guess-who session, and even some meditation! The best part was welcoming new and yet to join group members who are onboarding during this difficult time.
(Zoom screen! Megan, Aindrila, Javier, Jeff, Shweta, Deepanwita, Jing, Thomas, Anne, Russel. Aparajitha and Alex).
Nov 30th, 2020
The Daily Cal published a great article on our Pseudomonas putida study to require glucose use for indigoidine production!
Read it here Photo: Andrew, Thomas and Deepanwita
And Berkeley Lab IPO now has a link to the technology. See the Technology link here!
Oct 23th 2020
Product Substrate Pairing! This is great a start to what we hope are many more such systems to come. Being able to rewire metabolism to ensure that growth is coupled to the production of target compounds or their precursors allowed us to get the highest levels of indigoidine yet, in P. putida KT2440 from glucose. And the production shifted to an exponential phase. The nice production features held up in fed-batch and higher scales. Another collaboration with the ABPDU and JGI. Congrats to Deepanwita and Thomas for leading this paper, now out in Nat Comm.
August 13th 2020
Our paper on using the yeast Bar-seq library to study population dynamics during bioreactor cultivation is published. Congrats to Maren, Mitch, and Deepanwita. Great collaboration with the ABPDU. Check out the OA paper.
July 2nd 2020
Our paper on microbe-microbe interactions is out in Frontiers. We found iron to play an important role in antagonistic interactions, by using P. putida as a proxy microbe. Congrats to Thomas and Robin and collaboration with Jenny Mortimer!
March 24th 2020
Our first paper in Stay-at-Home life. A review on methods to study response regulators of Two component signaling systems. The paper is Open Access. We highlight the DAP-seq and DAP-chip methods developed in our group.
March 14th 2020
The day we left our labs for the shelter-in-place and then the stricter stay-at-home, as a response to COVID19. Many papers and proposals to write. And lots to pick up when we return.
Feb 24th 2020
Our study on a genome scale approach and implementation to engineer a P. putida strain that show high production reliably across scales in on BioRxiv.
Feb 13th 2020
Our 2020 annual m-group offsite. At the Cal Faculty Club again. A fantastic day of discussing science, careers, kindness, building clay objects and failing at unentangling human knots!!
Jan 11th 2020
Megan gives a beautiful talk on swapped domains in response regulators at the GRS for Sensory Transduction in Microorganisms at Ventura Beach, CA
Nov 21 2019
Thomas Eng and Yusuke Sasaki showed that ceratin variation in the heterologous isopentenol production pathway lead to the production of tetramethyl pyrazine – solely and upto 5g/L. TMP is famous for its use as a coffee flavoring! Open source in Metabolic Engineering Communications!
Nov 21 2019
Kudos to Ankita Kothari for showing that a putative mercury resistance operon present on an abundant 8-kbp native plasmid found in Oakridge ground waters encodes functional genes and that this plasmid can be taken up via natural competence by a range of isolates from this site! The paper is open access on mSystems.
Nov 21 2019
A very special day for our group. THREE papers accepted. One: Deepanwita and Aindrila co-authored a review led by Yan Chen and Chris Petzold on Systems biology methods and that’s accepted in Current Opinions in Biotechnology ! Thanks also to Deepanwita for supporting JBEI in our outreach activities (Nov 11th Biotechnology Celebration event on Saturday!)
Summer 2019: Conference season
We had a great summer of conferences! Thomas presented at the ECI conference, Ankita at the SEED conference Maren at the SIMB conference , Aindrila at Pseudomonas2019! We heard some great talks, got awesome feedback and came back to the lab inspired!
May 29th 2019
We engineered a fungal host, R. toruloides and optimized production condition to get high titer production of a blue pigment Indigoidine. This compound is a non-ribosomal peptide. We are now exploring if R. toruloides is a good host for other NRPs – a large group of compounds ranging from commodity to fine chemicals. All this and sustainability!! Read Maren’s open access paper here!
May 15th 2019
Our first paper on examining the impact of “JBEI” chemicals on biological systems is out! Robin and Thomas make a great team and we have fantastic cross-disciplinary contributors this story from across LBNL and also from external collaborators. We found that microbial presence does impact how plants respond to exposure to many chemicals. Check out the paper! More studies to follow and all in collaboration with the Mortimer group!!
May 3rd 2019
Very cool video of Ankita discussing Mercury as part of the #MyFavElement Series by Berkeley Lab! Check out the video here!
April 9th 2019
Lara’s paper is accepted! We have known since 2011 when we published our genome wide DAP-chip paperthat the Lur Regulon in Desulfovibrio vulgaris is targeted by several two component systems. We now show how LurR regulates this operon. Rajeev et al 2019
April 5th 2019
We got a chance to present our Host Engineering thoughts, ideas and stories at the DOE JGI User meeting in San Francisco! What an amazing group of speakers to be part of!
March 12th 2019
Aindrila presents the Plasmidome story in the IGI Seminar series and answers some cool questions for Mira Cheng’s blog. Mentions of the awesome Esther Lederberg and Mary-Dell Chilton were very well received! We also presented this work at the Technology Mini Colloquia at the the DOE Annual meeting in San Francisco on April 2nd!
Feb 27th 2019
We engineered Corynebactrium glutamicum to produce a pretty tidy level of isopentenol – a biofuel and a platform chemical. Great work by Yusuke Sasaki and Thomas Eng and the rest of the JBEI team! The paper is online and open access
Feb 26th 2019
Our discovery of large plasmids from the Oak Ridge FRC, spanning many incompatibility groups and many interesting functions is ONLINE!! Congratulations to Ankita Kothari and the team. Really pleased to have this out as it sets the stage for a lot more on this topic!
Feb 25th 2019
Our review on what we view to be challenges in engineering robust strains for large scale applications, and how Systems + Synthetic Biology can address them. Kudos to Maren for taking the lead on this! Read Online!!
Jan 17th 2019
2018 ended well for our team. We started the new year with an M-group annual offsite at the UC Faculty Club. It was a really fun day with interesting discussions on science, careers and some very fun team activities!
Dec 14th 2018
We published Maren’s study in characterizing the correct conditions required to produce the blue pigment Indigoidine in yeast. Also the first demonstration of this NRPS in Saccharomyces cerevisiae! Check out the paper
Dec 7th 2018
Congratulations to Jadie and Albert and their mentors Maren and Ankita for winning the best poster and best presentation competition. A special thanks to Drishti for her very cool poster also! And a shout out to the JBEI and ENIGMA projects!!
Oct 13th 2018
Our paper examining the role of an essential gene in improving not only tolerance but also restoring production levels in the presence of biomass pretreatment reagents is out in Microbial Cell Factories!! Check out the Open access paper here!
Jul 11th 2018
Proud of these two bright researchers going off to grad school this fall. Congratulations to Jess and Robin on their acceptance to Riverside and Cal!
Jan 17th 2018
Our study on two component signaling cross-regulation is out in Molecular Microbiology! We used the DAP-seq method that Megan has developed and presented at the recent Gordon Research Conference. Congratulations to Megan and the whole team!
Dec 8th 2017
Read Maren’s interview with BD Biosciences about the Accuri!
Nov 9th 2017
Oct 30th 2017
We published our paper in developing a synthetic transcriptional factor – hybrid promoter library for use in S. cerevisiae strain engineering.
Oct 12th 2017
Our study on improving membrane protein expression using genome wide edits is out in Scientific Reports!
July 17th 2017
DOE announces funding for JBEI other BRCs! Looking forward to many more years of research with a great team!
April 28th 2017
Duy presented her undergraduate work at the JBEI undergraduate Seminar! We loved having her in our group, and her research with Lara and Megan focused on understanding copper responsive systems in denitrifying Pseudomonads. She is a master of RNAseq preps! Congratulations on a great talk Duy!!
March 8th 2017
Ankita’s study on native plasmids from the Oakridge FRC is now online at BioRxiv!
Jan 19th 2017
Jan 5th 2017
Victor’s paper on Engineering glucose metabolism of Escherichia coli under nitrogen starvation is out in Nature Systems Biology and application.
November 27th 2016
May 10th 2016
Marijke’s paper on combining Ionic Liquid tolerance with biojet-fuel production is our in Green Chemistry and has a great write-up on Today at Berkeley Lab!
We lost a year of m-group news updates to grant writing…we may or may not be able to catch-up. But research was good and we published a lot!
August 17, 2015
Lara’s paper out in JBact. We described the role of a two component system in nitrite stress in the obligate anaerobe Desulfovibrio vulgaris.
July 3, 2015
The whole group at Bucci’s with all our visiting scientists and students (and the Bucci dog).
May 1, 2015
Victor’s study on a Limonene tolerant AhpC mutant is out in AEM. A good explanation for why Limonene is so much more toxic than other similar monoterpenes. Congratulations to Victor, Florence and the rest our team!!
April 17, 2015
Jan 20, 2015
Nov 4, 2014
We published our study on improving Isopentenol tolerance and production in the mBio journal. mBio featured it in their blog. We have gotten many more mentions (RDmag, Science Daily, Green car congress…). Congratulations to Heather, Jee Loon and the rest of the team!!
Feb 26, 2014
June 6, 2013
May 13, 2013
March 19, 2013
We get a mention in the Department Of Energy feature on STEM profiles.
Oct 1, 2012
The whole group at the Can’t Fail cafe. We have new members in the group – Amanda Reider Appel and Nhat Than Vuong!
Feb 14, 2012
Science Today, a UC Berkeley Radio channel, features comments on our study of the D. vulgaris two component system regulator network.
Nov 9, 2011
Oct 15, 2011
Lara and Eric’s D. vulgaris Response Regulator map paper is out in Genome Biology.
Sept 27, 2011
Mario (in the blue lab coat) features in the Berkeley Lab news letter TABL for his important contribution in developing Bisabolene production in yeast. Here is a link to Pamela and TaekSoon’s Nature Communications paper
August 22, 2011
July 1, 2011